Drinking Water Quality Microbiology Tests
Course Code: HS19
►Course Objective
- provide in-depth understanding of all key issues relevant to a proper estimation of the microbiological quality of water from different sources and different uses;
- equip participants with the tools needed to manage water quality effectively;
- familiarise participants with the latest practical laboratory methods and techniques for the detection of indicators and pathogens in water, and
- give a grounding in the function and use of Geographic Information Systems (GIS) in water quality management.
►Target Audience
- Engineers
- Goververment stuff
- Private company stuff which is related to water industry.
►Course Outline
Day 1
Talk 1
- Brief introduction of ABI
Talk 2
- Basic theory of Microbiology Analysis for water sample
Practical 1: Microbiology Analysis
- Media preparation and basic microbiology techniques
- Total Plate Count
- Detection of E.Coli and Coliform
- Detection of Salmonella
- Detection of Staphyllococcus aureus
Day 2
Talk 3
- Introduction of Kjeldahl method and Overview of Kjeltec 8200 for determination of nitrogen in water sample
Practical 1
- Determination of Nitrogen in drinking water
- Alkali solution preparation
- Receiver solution preparation
- Indicator solution preparation
- Scrubber unit solution preparation
Practical 2
- Determination of Nitrogen
- Digestion of sample using FOSS Tecator Digestion System
- Setting up FOSS Kjeltec Distillation Unit with solutions
Day 3
Practical 1
- Microbiology
- Enumeration of E.Coli and Coliform
- Detection of Salmonella sp. (cont.)
Practical 2
- Microbiology
- Enumeration of Salmonella sp.
- Detection of Staphyllococcus aureus (cont.)
Practical 3
- Determination of Nitrogen
- Titration of distillate
- Calculation of Nitrogen and presentation of results
Day 4
Talk 3
- Introduction and Overview of Inductively coupled plasma mass spectrometry (ICP-MS)
Practical 1
- Determination of heavy metal and mineral
- Sample preparation and digestion of sample in nitric acid with microwave digester
- Preparation of calibration standard solution for ICP-MS analysis
Practical 2
- Microbiology
- Enumeration of microorganism in Total Plate Count
- Enumeration of Staphyllococcus aureus
Practical 3
- Determination of heavy metal and mineral
- Familiarization with ICP-MS system and creating the project
Day 5
Practical 1
- Determination of heavy metal and mineral
- Run analysis of heavy metal and mineral using ICPMS
Practical 2
- Determination of heavy metal and mineral
- Plot calibration curve and perform data
- interpretation for heavy metal and mineral
- Q & A Session
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